IHC-P analysis of human kidney tissue using GTX83903 Profilin 1 antibody [1D5]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. Dilution : 1:50
![IHC-P analysis of human pancreas tissue using GTX83903 Profilin 1 antibody [1D5]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. Dilution : 1:50](https://www.genetex.com/upload/website/prouct_img/normal/GTX83903/GTX83903_1998_IHC-P_w_23061420_815.webp "IHC-P analysis of human pancreas tissue using GTX83903 Profilin 1 antibody [1D5]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. Dilution : 1:50")
![FACS analysis of HeLa cells using GTX83903 Profilin 1 antibody [1D5]. Red : Primary antibody Blue : Negative control antibody](https://www.genetex.com/upload/website/prouct_img/normal/GTX83903/GTX83903_229_FACS_w_23061420_527.webp "FACS analysis of HeLa cells using GTX83903 Profilin 1 antibody [1D5]. Red : Primary antibody Blue : Negative control antibody")
![FACS analysis of Jurkat cells using GTX83903 Profilin 1 antibody [1D5]. Red : Primary antibody Blue : Negative control antibody](https://www.genetex.com/upload/website/prouct_img/normal/GTX83903/GTX83903_230_FACS_w_23061420_660.webp "FACS analysis of Jurkat cells using GTX83903 Profilin 1 antibody [1D5]. Red : Primary antibody Blue : Negative control antibody")
![FACS analysis of HEK293T cells transfected with either Profilin 1 plasmid(Red) or empty vector control plasmid(Blue) using GTX83903 Profilin 1 antibody [1D5].](https://www.genetex.com/upload/website/prouct_img/normal/GTX83903/GTX83903_231_FACS_w_23061420_857.webp "FACS analysis of HEK293T cells transfected with either Profilin 1 plasmid(Red) or empty vector control plasmid(Blue) using GTX83903 Profilin 1 antibody [1D5].")
![WB analysis of various samples using GTX83903 Profilin 1 antibody [1D5]. Loading : 10 ug per lane Dilution : 1:200](https://www.genetex.com/upload/website/prouct_img/normal/GTX83903/GTX83903_3470_WB_w_23061420_766.webp "WB analysis of various samples using GTX83903 Profilin 1 antibody [1D5]. Loading : 10 ug per lane Dilution : 1:200")
![WB analysis of human tissues (Lane 1-Testis ; Lane 2-Omentum ; Lane 3-Uterus ; Lane 4-Breast ; Lane 5-Brain ; Lane 6-Liver ; Lane 7-Ovary ; Lane 8-Thyroid gland ; Lane 9-colon ; Lane 10-spleen) using GTX83903 Profilin 1 antibody [1D5]. Loading : 10 ug per lane Dilution : 1:200](https://www.genetex.com/upload/website/prouct_img/normal/GTX83903/GTX83903_3769_WB_w_23061420_195.webp "WB analysis of human tissues (Lane 1-Testis ; Lane 2-Omentum ; Lane 3-Uterus ; Lane 4-Breast ; Lane 5-Brain ; Lane 6-Liver ; Lane 7-Ovary ; Lane 8-Thyroid gland ; Lane 9-colon ; Lane 10-spleen) using GTX83903 Profilin 1 antibody [1D5]. Loading : 10 ug per lane Dilution : 1:200")
![WB analysis of HEK293T cells transfected with Profilin 1 plasmid (Right) or empty vector (Left) for 48 hrs using GTX83903 Profilin 1 antibody [1D5]. Loading : 5 ug per lane](https://www.genetex.com/upload/website/prouct_img/normal/GTX83903/GTX83903_3982_WB_w_23061420_372.webp "WB analysis of HEK293T cells transfected with Profilin 1 plasmid (Right) or empty vector (Left) for 48 hrs using GTX83903 Profilin 1 antibody [1D5]. Loading : 5 ug per lane")
![WB analysis of various cell lines using GTX83903 Profilin 1 antibody [1D5]. Loading : 35 ug per lane](https://www.genetex.com/upload/website/prouct_img/normal/GTX83903/GTX83903_3984_WB_w_23061420_102.webp "WB analysis of various cell lines using GTX83903 Profilin 1 antibody [1D5]. Loading : 35 ug per lane")
![ICC/IF analysis of COS7 cells transiently transfected with Profilin 1 plasmid using GTX83903 Profilin 1 antibody [1D5].](https://www.genetex.com/upload/website/prouct_img/normal/GTX83903/GTX83903_869_ICCIF_w_23061420_175.webp "ICC/IF analysis of COS7 cells transiently transfected with Profilin 1 plasmid using GTX83903 Profilin 1 antibody [1D5].")
IHC-P analysis of human kidney tissue using GTX83903 Profilin 1 antibody [1D5]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. Dilution : 1:50
Profilin 1 antibody [1D5]
GTX83903
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetPFN1
Overview
- SupplierGeneTex
- Product NameProfilin 1 antibody [1D5] - Overexpression Validated
- Delivery Days Customer9
- Application Supplier NoteWB: 1:200-1:2000. ICC/IF: 1:100. IHC-P: 1:50. FACS: 1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID1D5
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID5216
- Target namePFN1
- Target descriptionprofilin 1
- Target synonymsALS18, profilin-1, epididymis tissue protein Li 184a, profilin I
- HostMouse
- IsotypeIgG2a
- Protein IDP07737
- Protein NameProfilin-1
- Scientific DescriptionBinds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG.
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
Datasheet
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![IHC-P analysis of human kidney tissue using GTX83902 Profilin 1 antibody [2D2]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. Dilution : 1:50](https://www.genetex.com/upload/website/prouct_img/normal/GTX83902/GTX83902_1994_IHC-P_w_23061420_907.webp)
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References
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