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ProductsBack/Profilin 1 (PFN1) Mouse Monoclonal Antibody [Clone ID: OTI2D2]
Figure A, Western blot analysis of overexpressed lysates (5ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, NC), human PFN1 plasmid (RC202338, hPFN1), mouse PFN1 plasmid (MR200895, mPFN1) using anti-PFN1 antibody TA501243 (1:10000@1mg/ml). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (TA180144, 1:10000@1mg/ml).
Figure A, Western blot analysis of overexpressed lysates (5ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, NC), human PFN1 plasmid (RC202338, hPFN1), mouse PFN1 plasmid (MR200895, mPFN1) using anti-PFN1 antibody TA501243 (1:10000@1mg/ml). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (TA180144, 1:10000@1mg/ml).
Figure A, Western blot analysis of overexpressed lysates (5ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, NC), human PFN1 plasmid (RC202338, hPFN1), mouse PFN1 plasmid (MR200895, mPFN1) using anti-PFN1 antibody TA501243 (1:10000@1mg/ml). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (TA180144, 1:10000@1mg/ml).

Profilin 1 (PFN1) Mouse Monoclonal Antibody [Clone ID: OTI2D2]

CF501243
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityCanine, Human, Monkey, Mouse, Rat
TargetPFN1
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    Profilin 1 (PFN1) Mouse Monoclonal Antibody [Clone ID: OTI2D2]
  • Delivery Days Customer
    14
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI2D2
  • Gene ID5216
  • Target name
    PFN1
  • Target description
    profilin 1
  • Target synonyms
    ALS18, profilin-1, epididymis tissue protein Li 184a, profilin I
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP07737
  • Protein Name
    Profilin-1
  • Scientific Description
    Carrier-free (BSA/glycerol-free) PFN1 mouse monoclonal antibody, clone OTI2D2 (formerly 2D2)
  • Reactivity
    Canine, Human, Monkey, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of Profilin1 using anti-Profilin1 antibody (PB9313). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: rat brain tissue lysates, Lane 7: mouse testis tissue lysates, Lane 8: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Profilin1 antigen affinity purified polyclonal antibody (Catalog # PB9313) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Profilin1 at approximately 15 kDa. The expected band size for Profilin1 is at 15 kDa.
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