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WB analysis of various sample lysates using GTX33429 Profilin 2 antibody. The signal was developed with ECL plus-Enhanced. Dilution : 1:500 Loading : 25microg per lane
WB analysis of various sample lysates using GTX33429 Profilin 2 antibody. The signal was developed with ECL plus-Enhanced. Dilution : 1:500 Loading : 25microg per lane
WB analysis of various sample lysates using GTX33429 Profilin 2 antibody. The signal was developed with ECL plus-Enhanced. Dilution : 1:500 Loading : 25microg per lane

Profilin 2 antibody

GTX33429
GeneTex
ApplicationsWestern Blot
DownloadDatasheet
Product group Antibodies
TargetPFN2
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Overview

  • Supplier
    GeneTex
  • Product Name
    Profilin 2 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500 - 1:2000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5217
  • Target name
    PFN2
  • Target description
    profilin 2
  • Target synonyms
    D3S1319E; PFL; profilin II; profilin-2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP35080
  • Protein Name
    Profilin-2
  • Scientific Description
    The protein encoded by this gene is a ubiquitous actin monomer-binding protein belonging to the profilin family. It is thought to regulate actin polymerization in response to extracellular signals. There are two alternatively spliced transcript variants encoding different isoforms described for this gene. [provided by RefSeq, Jul 2008]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Profilin 2/PFN2 using anti-Profilin 2/PFN2 antibody (A06663). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human U87 whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human Hela whole cell lysates, Lane 5: human HEK293 whole cell lysates, Lane 6: rat brain tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse kidney tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Profilin 2/PFN2 antigen affinity purified polyclonal antibody (Catalog # A06663) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Profilin 2/PFN2 at approximately 15KD. The expected band size for Profilin 2/PFN2 is at 15KD.
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