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ProductsBack/Prolactin (PRL) Mouse Monoclonal Antibody [Clone ID: OTI9D12]
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PRL (RC205627, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PRL. Positive lysates LY424443 (100ug) and LC424443 (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PRL (RC205627, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PRL. Positive lysates LY424443 (100ug) and LC424443 (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PRL (RC205627, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PRL. Positive lysates LY424443 (100ug) and LC424443 (20ug) can be purchased separately from OriGene.

Prolactin (PRL) Mouse Monoclonal Antibody [Clone ID: OTI9D12]

CF500718
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot
Product group Antibodies
ReactivityHuman
TargetPRL
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    Prolactin (PRL) Mouse Monoclonal Antibody [Clone ID: OTI9D12]
  • Delivery Days Customer
    14
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI9D12
  • Gene ID5617
  • Target name
    PRL
  • Target description
    prolactin
  • Target synonyms
    GHA1, prolactin, decidual prolactin, growth hormone A1
  • Host
    Mouse
  • Isotype
    IgG2b
  • Protein IDP01236
  • Protein Name
    Prolactin
  • Scientific Description
    Carrier-free (BSA/glycerol-free) PRL mouse monoclonal antibody, clone OTI9D12 (formerly 9D12)
  • Reactivity
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Prolactin/PRL using anti-Prolactin/PRL antibody (A00601-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Prolactin/PRL antigen affinity purified polyclonal antibody (Catalog # A00601-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Prolactin/PRL at approximately 26 kDa. The expected band size for Prolactin/PRL is at 26 kDa.
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