Bio-Connect

Pronase reagent (Ready-to-use)

Research Use Only
GTX73181
GeneTex
Product group Molecular Biology
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Overview

  • Supplier
    GeneTex
  • Product Name
    Pronase reagent
  • Delivery Days Customer
    9
  • Application Supplier Note
    For IHC: Use as antigen retriever for some antibodies in IHC. Reagent is supplied in ready-to-use format. Over-digestion may detach tissue sections from slides or may result in the loss of tissue morphology. It may be necessary to use + charge, sialanized or polylysine coated slides.
  • Certification
    Research Use Only
  • Formulation
    Liquid
  • Scientific Description
    Pronase is used for photolytic digestion of formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies. In IHC most commonly used fixative like formalin mask tissue antigens (cellular, membrane and nuclear) by cross-linking process, this results in poor or no staining in IHC. Pronase digestion of FFPE tissue section improves accessibility of antibodies to tissue antigens.
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352204

References

  • Selective tumor hypoxia targeting by hypoxia-activated prodrug TH-302 inhibits tumor growth in preclinical models of cancer. Sun JD et al., 2012 Feb 1, Clin Cancer Res
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