Pronase reagent (Ready-to-use)
GTX73181
Product group Molecular Biology
Overview
- SupplierGeneTex
- Product NamePronase reagent
- Delivery Days Customer9
- Application Supplier NoteFor IHC: Use as antigen retriever for some antibodies in IHC. Reagent is supplied in ready-to-use format. Over-digestion may detach tissue sections from slides or may result in the loss of tissue morphology. It may be necessary to use + charge, sialanized or polylysine coated slides.
- CertificationResearch Use Only
- FormulationLiquid
- Scientific DescriptionPronase is used for photolytic digestion of formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies. In IHC most commonly used fixative like formalin mask tissue antigens (cellular, membrane and nuclear) by cross-linking process, this results in poor or no staining in IHC. Pronase digestion of FFPE tissue section improves accessibility of antibodies to tissue antigens.
- Storage Instruction2°C to 8°C
- UNSPSC12352204
References
- Selective tumor hypoxia targeting by hypoxia-activated prodrug TH-302 inhibits tumor growth in preclinical models of cancer. Sun JD et al., 2012 Feb 1, Clin Cancer ResRead more