Propidium Iodide Buffer [25535-16-4]

Biotium

Propidium Iodide Buffer

9

Flow Cytometry, Other Application

25535-16-4

Research Use Only

Propidium iodide (PI) is a cell impermeable nucleic acid intercalating dye. Because PI is excluded from viable cells it can be used in apoptotic studies to distinguish necrotic cells or cells in the late stages of apoptosis from viable cells or cells in early apoptosis where cell membranes are still intact. PI is also utilized as a counterstain in multicolor fluorescent imaging. After entering cells and binding to DNA and RNA, the fluorescence of PI is enhanced 20- to 30-fold. PI can be used in flow cytometry and fluorescence microscopy. 50 ug/mL solution in PBS buffer Orange / red liquid Store at 4°C and protect from light lambdaEx/lambdaEm (DNA-bound) = 535/617 nm lambdaEx/lambdaEm (no DNA) = 493/636 nm C27H34I2N4 MW: 668.4 [25535-16-4]

Synthetic

2°C to 8°C

12161700