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Prostate Specific Antigen Antibody (YA1537, Rabbit)

HY-P81792
MedChem Express
TargetKLK3
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    Prostate Specific Antigen Antibody (YA1537, Rabbit)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID354
  • Target name
    KLK3
  • Target description
    kallikrein related peptidase 3
  • Target synonyms
    APS, KLK2A1, PSA, hK3, prostate-specific antigen, P-30 antigen, gamma-seminoprotein, kallikrein-3, semenogelase, seminin
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    Prostate Specific Antigen Antibody (YA1537) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA1537), targeting Prostate Specific Antigen, with a predicted molecular weight of 29 kDa (observed band size: 34 kDa). Prostate Specific Antigen Antibody (YA1537) can be used for WB, IHC-P experiment in human background.
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Prostate Specific Antigen/KLK3 using anti-Prostate Specific Antigen/KLK3 antibody (A01505-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human LNCAP whole cell lysates, Lane 2: human LNCAP whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Prostate Specific Antigen/KLK3 antigen affinity purified polyclonal antibody (Catalog # A01505-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Prostate Specific Antigen/KLK3 at approximately 34KD. The expected band size for Prostate Specific Antigen/KLK3 is at 34KD.
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