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ProductsBack/Protein Tyrosine Phosphatase, Non Receptor Type 13 (PTPN13) Polyclonal Antibody

Protein Tyrosine Phosphatase, Non Receptor Type 13 (PTPN13) Polyclonal Antibody

CAU23254
Biomatik
ApplicationsWestern Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetPTPN13
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Overview

  • Supplier
    Biomatik
  • Product Name
    Protein Tyrosine Phosphatase, Non Receptor Type 13 (PTPN13) Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB; ICC; IHC-P; IHC-F; ELISA.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID5783
  • Target name
    PTPN13
  • Target description
    protein tyrosine phosphatase non-receptor type 13
  • Target synonyms
    FAP-1, PNP1, PTP-BAS, PTP-BL, PTP1E, PTPL1, PTPLE, hPTP1E, tyrosine-protein phosphatase non-receptor type 13, fas-associated protein-tyrosine phosphatase 1, hPTPE1, protein tyrosine phosphatase, non-receptor type 13 (APO-1/CD95 (Fas)-associated phosphatase), protein-tyrosine phosphatase 1E, protein-tyrosine phosphatase PTPL1
  • Host
    Rabbit
  • Protein IDQ12923
  • Protein Name
    Tyrosine-protein phosphatase non-receptor type 13
  • Scientific Description
    The Protein Tyrosine Phosphatase, Non Receptor Type 13 (PTPN13) Polyclonal Antibody (Species: Human) has been validated for the following applications: WB, ICC, IHC-P, IHC-F, ELISA.
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PTPN13 using anti-PTPN13 antibody (A02481-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTPN13 antigen affinity purified polyclonal antibody (Catalog # A02481-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PTPN13 at approximately 270 kDa. The expected band size for PTPN13 is at 270 kDa.
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