Bio-Connect

Proteins / Signaling Molecules

This product group contains both native proteins and recombinant proteins expressed and purified from bacterial, insect and mammalian expression systems. Bio-Connect also offers fusion proteins, peptides, enzymes, bacterial and viral proteins, hormones and growth factors. Furthermore, it includes chemokines and cytokines, collagenases, albumins, globulins, blood/plasma related proteins and more.

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Product group Proteins / Signaling Molecules
GeneTex
ApplicationsFunctional Assay
  • SizePrice
Purity of recombinant rat Visfatin (2 microg) was analyzed by SDS-PAGE electrphoresis.
Product group Proteins / Signaling Molecules
GeneTex
ApplicationsFunctional Assay
  • SizePrice
Product group Proteins / Signaling Molecules
GeneTex
ApplicationsFunctional Assay
  • SizePrice
Product group Proteins / Signaling Molecules
GeneTex
ApplicationsFunctional Assay
  • SizePrice
Product group Proteins / Signaling Molecules
GeneTex
ApplicationsFunctional Assay
  • SizePrice
Product group Proteins / Signaling Molecules
GeneTex
ApplicationsFunctional Assay
  • SizePrice
GTX65629-pro GITRL protein induced GITR-mediated NF-kappaB activation. HEK293 cells were transiently transfected with 20ng of a (NF-kappaB)-Luc reporter, 2ng of a Renilla luciferase, and with 20ng of a GITR full length encoding construct. At 24hr after transfection, the indicated amounts of GITRL protein or a DDDDK control protein were treated for 4hr and followed by the measurement of dual luciferase activities.
Product group Proteins / Signaling Molecules
GeneTex
ApplicationsFunctional Assay
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GTX65630-pro Salmonella typhimurium Flagellin protein activates TLR5 in vivo. Mice WT are injected i.v. with indicated doses of Flagellin protein. After 2 hours, levels of CCL20 and IL-6 in the serum were measured by ELISA. As a control, mice TLR5 -/- have been injected i.v. with 10microg of Flagellin protein, but no expression of CCL20 and IL-6 could be observed (not shown).
Product group Proteins / Signaling Molecules
GeneTex
ApplicationsFunctional Assay
  • SizePrice
Binding of GTX65631-pro IL2 (Superkine) human Fc fusion protein to IL-2Rbeta (human) is increased >10 fold compared to IL-2 human Fc fusion protein. Method: IL-2Rbeta (human) protein was coated on an ELISA plate at 1microg/ml. After blocking and washing steps, indicated concentrations of GTX65631-pro IL-2 Superkine (Fc) or IL-2 (Fc) protein were added. Following incubation for 1 h at RT, the binding was detected using an anti-human Fc antibody (HRP).
Product group Proteins / Signaling Molecules
GeneTex
ApplicationsFunctional Assay
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GTX65633-pro CD137L protein (DDDDK tagged) binding to CD137 transfected cells. HEK 293 cells were transiently transfected with a construct encoding full-length mouse CD137. At 48hrs after transfection, the transfectants were incubated with 0.5 or 5microg/ml GTX65633 CD137L protein (DDDDK tagged) and followed by flow cytometry analysis with anti-DDDDK anibody.
Product group Proteins / Signaling Molecules
GeneTex
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Pull down assay of GTX65634-pro CD137L protein (DDDDK tagged) by hCD137-Fc. 1. hCD137-Fc + hCD137L-DDDDDK-tag 2. hCD137-Fc + hGITRL-DDDDDK-tag 3. hCD137-Fc + hGITRL-His-tag 4. hGITR-Fc + hCD137L-DDDDDK-tag 5. hGITR-Fc + hGITRL-DDDDDK-tag
Product group Proteins / Signaling Molecules
GeneTex
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GTX65635-pro CD40L protein (multimeric) does not need an enhancer to induce B cells activation. Method: PBL cells were incubated in 96-well plates (2x105 cells/well in 100microl RPMI supplemented with 10% FCS) for 24 hours at 37oC with the indicated concentration of CD40L (multimeric) or CD40L in the presence and absence of 1microg/ml Enhancer. Cells were washed with PBS and stained with CD86-PE antibody and CD19-FITC antibody, and then analyzed by flow cytometry.
Product group Proteins / Signaling Molecules
GeneTex
ApplicationsFunctional Assay
  • SizePrice