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ProductsBack/Protor 1 (PRR5) Rabbit Polyclonal Antibody
WB Suggested Anti-PRR5 Antibody Titration: 0.2-1 ug/ml
Positive Control: COLO205 cell lysatePRR5 is supported by BioGPS gene expression data to be expressed in COLO205
WB Suggested Anti-PRR5 Antibody Titration: 0.2-1 ug/ml<br/>Positive Control: COLO205 cell lysatePRR5 is supported by BioGPS gene expression data to be expressed in COLO205
WB Suggested Anti-PRR5 Antibody Titration: 0.2-1 ug/ml
Positive Control: COLO205 cell lysatePRR5 is supported by BioGPS gene expression data to be expressed in COLO205

Protor 1 (PRR5) Rabbit Polyclonal Antibody

TA357331
OriGene
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
Price on request
100 ul
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Overview

  • Supplier
    OriGene
  • Product Name
    Protor 1 (PRR5) Rabbit Polyclonal Antibody
  • Delivery Days Customer
    14
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Host
    Rabbit
  • Protein IDB3KRM4
  • Protein Name
    cDNA FLJ34565 fis, clone KIDNE2006210, highly similar to Homo sapiens proline rich protein 5 (PRR5), transcript variant 1, mRNA
  • Scientific Description
    PRR5 Antibody - N-terminal region
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of PRR5 using anti-PRR5 antibody (A05975-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRR5 antigen affinity purified polyclonal antibody (Catalog # A05975-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRR5 at approximately 39 kDa. The expected band size for PRR5 is at 33,43,50 kDa.
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