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PROX1 antibody

10R-8376
Biosynth
Product group Antibodies
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Overview

  • Supplier
    Biosynth
  • Product Name
    PROX1 antibody
  • Delivery Days Customer
    11
  • Certification
    Research Use Only
  • UNSPSC
    41116161

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The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using CSB-PA215840(PROX1 Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: x200)
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Figure 1. Western blot analysis of PROX1 using anti-PROX1 antibody (A01985-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human PC-3 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PROX1 antigen affinity purified polyclonal antibody (Catalog # A01985-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PROX1 at approximately 90 kDa. The expected band size for PROX1 is at 83 kDa.
Product group Antibodies
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Anti-PROX1 Antibody Picoband(r)A01985-1-CARRIER-FREE
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RayBiotech
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Immunohistochemical staining of mouse hippocampus shows strong nuclear positivity in the granular cell layer of the dentate gyrus.
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K562 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20℃, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with prox1 Antibody(bs-2774R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Product group Antibodies
References
Bioss
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GeneTex
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