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ProductsBack/PRP6 antibody [C1C3]
Sample (30 ug of whole cell lysate) A: HCT116 7.5% SDS PAGE GTX115765 diluted at 1:1000
Sample (30 ug of whole cell lysate) A: HCT116 7.5% SDS PAGE GTX115765 diluted at 1:1000
Sample (30 ug of whole cell lysate) A: HCT116 7.5% SDS PAGE GTX115765 diluted at 1:1000

PRP6 antibody [C1C3]

GTX115765
GeneTex
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetPRPF6
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Overview

  • Supplier
    GeneTex
  • Product Name
    PRP6 antibody [C1C3]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.8 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID24148
  • Target name
    PRPF6
  • Target description
    pre-mRNA processing factor 6
  • Target synonyms
    ANT-1, ANT1, C20orf14, Prp6, RP60, SNRNP102, TOM, U5-102K, hPrp6, pre-mRNA-processing factor 6, PRP6 homolog, PRP6 pre-mRNA processing factor 6 homolog, U5 snRNP-associated 102 kDa protein, U5-102 kDa protein, androgen receptor N-terminal domain transactivating protein-1, p102 U5 small nuclear ribonucleoprotein particle-binding protein, putative mitochondrial outer membrane protein import receptor
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO94906
  • Protein Name
    Pre-mRNA-processing factor 6
  • Scientific Description
    The protein encoded by this gene appears to be involved in pre-mRNA splicing, possibly acting as a bridging factor between U5 and U4/U6 snRNPs in formation of the spliceosome. The encoded protein also can bind androgen receptor, providing a link between transcriptional activation and splicing. [provided by RefSeq]
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PRPF6 using anti-PRPF6 antibody (A06724-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: rat C6 whole cell lysates, Lane 5: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRPF6 antigen affinity purified polyclonal antibody (Catalog # A06724-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRPF6 at approximately 107 kDa. The expected band size for PRPF6 is at 107 kDa.
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