More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/PRP8 antibody [C3-2], C-term

PRP8 antibody [C3-2], C-term

GTX108046
GeneTex
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetPRPF8
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    GeneTex
  • Product Name
    PRP8 antibody [C3-2], C-term
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID10594
  • Target name
    PRPF8
  • Target description
    pre-mRNA processing factor 8
  • Target synonyms
    HPRP8, PRP8, PRPC8, RP13, SNRNP220, pre-mRNA-processing-splicing factor 8, 220 kDa U5 snRNP-specific protein, PRP8 homolog, PRP8 pre-mRNA processing factor 8 homolog, U5 snRNP-specific protein (220 kD), ortholog of S. cerevisiae Prp8p, apoptosis-regulated protein 1, apoptosis-regulated protein 2, p220, precursor mRNA processing protein, splicing factor Prp8
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ6P2Q9
  • Protein Name
    Pre-mRNA-processing-splicing factor 8
  • Scientific Description
    Pre-mRNA splicing occurs in 2 sequential transesterification steps. The protein encoded by this gene is a component of both U2- and U12-dependent spliceosomes, and found to be essential for the catalytic step II in pre-mRNA splicing process. It contains several WD repeats, which function in protein-protein interactions. This protein has a sequence similarity to yeast Prp8 protein. This gene is a candidate gene for autosomal dominant retinitis pigmentosa. [provided by RefSeq]
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Moriel-Carretero M, Ovejero S, Gérus-Durand M, et al. Fanconi anemia FANCD2 and FANCI proteins regulate the nuclear dynamics of splicing factors. J Cell Biol. 2017,216(12):4007-4026. doi: 10.1083/jcb.201702136
    Read this paper
  • Ameyar-Zazoua M, Rachez C, Souidi M, et al. Argonaute proteins couple chromatin silencing to alternative splicing. Nat Struct Mol Biol. 2012,19(10):998-1004. doi: 10.1038/nsmb.2373
    Read this paper

Datasheet

Download Datasheet

FAQ

Go to our frequently asked questions

Related products

Product group Antibodies
Bioss
PRPF8 Recombinant Antibody, AbBy Fluor-647 ConjugatedBSM-61967R-BF647
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
ReactivityHuman, Mouse, Rat
TargetPRPF8
  • SizePrice
Product group Antibodies
Assay Genie
PRPF8 Monoclonal AntibodyCAB4575
ReactivityHuman
TargetPRPF8
  • SizePrice
Product group Antibodies
RayBiotech
Anti-PRPF8 Antibody144-06053
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetPRPF8
  • SizePrice
WB analysis of various sample lysates using GTX65909 PRP8 antibody. Dilution : 1:1000 Loading : 25microg per lane
Product group Antibodies
GeneTex
PRP8 antibodyGTX65909
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse, Rat
TargetPRPF8
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
PRPF8 AntibodyLS-C829997
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse
TargetPRPF8
  • SizePrice
Product group Antibodies
MedChem Express
PRPF8 Antibody (YA1759, Rabbit)HY-P82014
TargetPRPF8
  • SizePrice
Immunofluorescence staining of Hela cells with CSB-PA744036LA01HU at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Product group Antibodies
Cusabio
PRPF8 AntibodyCSB-PA744036LA01HU
ApplicationsImmunoFluorescence, ELISA
ReactivityHuman
TargetPRPF8
  • SizePrice
Figure 1. Western blot analysis of PRPF8 using anti-PRPF8 antibody (A02878-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRPF8 antigen affinity purified polyclonal antibody (Catalog # A02878-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRPF8 at approximately 250 kDa. The expected band size for PRPF8 is at 274 kDa.
Product group Antibodies
Boster Bio
Anti-PRPF8 Antibody Picoband(r)A02878-2-CARRIER-FREE
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse
TargetPRPF8
  • SizePrice