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Western Blot analysis of HuvEc cells using PRPF18 Polyclonal Antibody
Western Blot analysis of HuvEc cells using PRPF18 Polyclonal Antibody
Western Blot analysis of HuvEc cells using PRPF18 Polyclonal Antibody

PRPF18 Antibody

CSB-PA003856
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetPRPF18
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Overview

  • Supplier
    Cusabio
  • Product Name
    PRPF18 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID8559
  • Target name
    PRPF18
  • Target description
    pre-mRNA processing factor 18
  • Target synonyms
    hPrp18; pre-mRNA-splicing factor 18; PRP18; PRP18 homolog; PRP18 pre-mRNA processing factor 18 homolog; PRP18 pre-mRNA processing factor 18 homolog(PRPF18)
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ99633
  • Protein Name
    Pre-mRNA-splicing factor 18
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PRPF18 using anti-PRPF18 antibody (A13674-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: rat brain tissue lysates, Lane 3: rat PC-12 whole cell lysates, Lane 4: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRPF18 antigen affinity purified polyclonal antibody (Catalog # A13674-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRPF18 at approximately 42 kDa. The expected band size for PRPF18 is at 40,42 kDa.
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