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PRR5 antibody

ORB541151
Biorbyt
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetPRR5
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Overview

  • Supplier
    Biorbyt
  • Product Name
    PRR5 antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB: 1:500-1:2000, IHC-P: 1:50-1:200, IF: 1:50-1:200 ELISA, IF, WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID55615
  • Target name
    PRR5
  • Target description
    proline rich 5
  • Target synonyms
    FLJ20185k, PP610, PROTOR-1, PROTOR1, proline-rich protein 5, Rho GTPase activating protein 8, proline rich 5 (renal), protein observed with Rictor-1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP85299
  • Protein Name
    Proline-rich protein 5
  • Scientific Description
    Rabbit polyclonal antibody to PRR5
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PRR5 using anti-PRR5 antibody (A05975-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRR5 antigen affinity purified polyclonal antibody (Catalog # A05975-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRR5 at approximately 39 kDa. The expected band size for PRR5 is at 33,43,50 kDa.
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