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Immunohistochemistry of paraffin-embedded human lung cancer using CSB-PA018830LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human lung cancer using CSB-PA018830LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human lung cancer using CSB-PA018830LA01HU at dilution of 1:100

PRTN3 Antibody

CSB-PA018830LA01HU
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetPRTN3
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Overview

  • Supplier
    Cusabio
  • Product Name
    PRTN3 Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5657
  • Target name
    PRTN3
  • Target description
    proteinase 3
  • Target synonyms
    ACPA, AGP7, C-ANCA, CANCA, MBN, MBT, NP-4, NP4, P29, PR-3, PR3, myeloblastin, C-ANCA antigen, Wegener granulomatosis autoantigen, azurophil granule protein 7, leukocyte proteinase 3, neutrophil proteinase 4, serine proteinase, neutrophil, wegener autoantigen
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP24158
  • Protein Name
    Myeloblastin
  • Scientific Description
    Polymorphonuclear leukocyte serine protease that degrades elastin, fibronectin, laminin, vitronectin, and collagen types I, III, and IV (in vitro) and causes emphysema when administered by tracheal insufflation to hamsters.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PRTN3 using anti-PRTN3 antibody (A03863-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRTN3 antigen affinity purified polyclonal antibody (Catalog # A03863-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRTN3 at approximately 32 kDa. The expected band size for PRTN3 is at 28,32 kDa.
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