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Western Blot Positive WB detected in: HepG2 whole cell lysate, MCF-7 whole cell lysate, Jurkat whole cell lysate All lanes: PRUNE1 antibody at 4microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 51, 43, 31, 27, 25, 19 kDa Observed band size: 60 kDa
Western Blot Positive WB detected in: HepG2 whole cell lysate, MCF-7 whole cell lysate, Jurkat whole cell lysate All lanes: PRUNE1 antibody at 4microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 51, 43, 31, 27, 25, 19 kDa Observed band size: 60 kDa
Western Blot Positive WB detected in: HepG2 whole cell lysate, MCF-7 whole cell lysate, Jurkat whole cell lysate All lanes: PRUNE1 antibody at 4microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 51, 43, 31, 27, 25, 19 kDa Observed band size: 60 kDa

PRUNE1 Antibody

CSB-PA771430LA01HU
Cusabio
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetPRUNE1
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Overview

  • Supplier
    Cusabio
  • Product Name
    PRUNE1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID58497
  • Target name
    PRUNE1
  • Target description
    prune exopolyphosphatase 1
  • Target synonyms
    DRES-17, DRES17, H-PRUNE, HTCD37, NMIHBA, PRUNE, exopolyphosphatase PRUNE1, Drosophila-related expressed sequence 17, protein prune homolog 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ86TP1
  • Protein Name
    Exopolyphosphatase PRUNE1
  • Scientific Description
    Phosphodiesterase (PDE) that has higher activity toward cAMP than cGMP, as substrate. Plays a role in cell proliferation, migration and differentiation, and acts as a negative regulator of NME1. Plays a role in the regulation of neurogenesis (PubMed:28334956). Involved in the regulation of microtubule polymerization (PubMed:28334956).
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PRUNE1 using anti-PRUNE1 antibody (A31798). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human HEL whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRUNE1 antigen affinity purified polyclonal antibody (Catalog # A31798) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRUNE1 at approximately 60 kDa. The expected band size for PRUNE1 is at 50,60 kDa.
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