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ProductsBack/PRX I antibody [N1C2]

PRX I antibody [N1C2]

GTX101705
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
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Product group Antibodies
TargetPRDX1
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Overview

  • Supplier
    GeneTex
  • Product Name
    PRX I antibody [N1C2]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.22 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID5052
  • Target name
    PRDX1
  • Target description
    peroxiredoxin 1
  • Target synonyms
    epididymis secretory sperm binding protein; MSP23; natural killer cell-enhancing factor A; natural killer-enhancing factor A; NKEFA; NKEF-A; PAG; PAGA; PAGB; peroxiredoxin-1; proliferation-associated gene A; proliferation-associated gene protein; PRX1; PRXI; TDPX2; thioredoxin peroxidase 2; thioredoxin-dependent peroxide reductase 2; thioredoxin-dependent peroxiredoxin 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ06830
  • Protein Name
    Peroxiredoxin-1
  • Scientific Description
    This gene encodes a member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. The encoded protein may play an antioxidant protective role in cells, and may contribute to the antiviral activity of CD8(+) T-cells. This protein may have a proliferative effect and play a role in cancer development or progression. Three transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Pomegranate Extract (POMx) Induces Mitochondrial Dysfunction and Apoptosis of Oral Cancer Cells. Peng SY et al., 2021 Jul 13, Antioxidants (Basel)
    Read more
  • The Long-Term DEHP Exposure Confers Multidrug Resistance of Triple-Negative Breast Cancer Cells through ABC Transporters and Intracellular ROS. Jadhao M et al., 2021 Jun 11, Antioxidants (Basel)
    Read more
  • PRDX1 activates autophagy via the PTEN-AKT signaling pathway to protect against cisplatin-induced spiral ganglion neuron damage. Liu W et al., 2021 Dec, Autophagy
    Read more
  • The Phenoxyphenol Compound 4-HPPP Selectively Induces Antiproliferation Effects and Apoptosis in Human Lung Cancer Cells through Aneupolyploidization and ATR DNA Repair Signaling. Liu W et al., 2020, Oxid Med Cell Longev
    Read more
  • Effect of glutathione reductase knockdown in response to UVB-induced oxidative stress in human lung adenocarcinoma. Chou HC et al., 2014 Jan 9, Proteome Sci
    Read more
  • 5-Methoxytryptophan-dependent protection of cardiomyocytes from heart ischemia reperfusion injury. Chou HC et al., 2014 Feb 1, Arch Biochem Biophys
    Read more
  • Proteomic and redox-proteomic study on the role of glutathione reductase in human lung cancer cells. Fan CY et al., 2013 Dec, Electrophoresis
    Read more
  • Peroxiredoxin 1 is a tumor-associated antigen in esophageal squamous cell carcinoma. Ren P et al., 2013 Nov, Oncol Rep
    Read more

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Figure 1. Western blot analysis of Peroxiredoxin 1 using anti-Peroxiredoxin 1 antibody (PB9348). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human MDA-MB-453 whole cell lysates, Lane 4: human Hela whole cell lysates, Lane 5: human HepG2 whole cell lysates, Lane 6: human HEK293 whole cell lysates, Lane 7: human placenta tissue lysates, Lane 8: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Peroxiredoxin 1 antigen affinity purified polyclonal antibody (Catalog # PB9348) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Peroxiredoxin 1 at approximately 24 kDa. The expected band size for Peroxiredoxin 1 is at 22 kDa.
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