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PRX / Periaxin Antibody

LS-C662559
LifeSpan BioSciences
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
TargetPRX
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    PRX / Periaxin Antibody
  • Delivery Days Customer
    23
  • Applications
    Flow Cytometry, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Applications Supplier
    ELISA, Flo, ICC, IHC, WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID57716
  • Target name
    PRX
  • Target description
    periaxin
  • Target synonyms
    CMT4F; periaxin
  • Host
    Rabbit
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PRX using anti-PRX antibody (A01686). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: humna U2OS whole cell lysates, Lane 4: human U251 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRX antigen affinity purified polyclonal antibody (Catalog # A01686) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRX at approximately 155 kDa. The expected band size for PRX is at 148 kDa.
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