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PSEN2 / Presenilin 2 Antibody

LS-C26150
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetPSEN2
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    PSEN2 / Presenilin 2 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    Can be used at 1:1000 to 1:4000 for both ELISA and immunoblot testing. User needs to try out the titration for immunohistochemical testing. To minimize background staining, we suggest using higher content of detergent (Tween-20) in dilution the Abs and washing.. ELISA, IHC, WB Can be used at 1:1000 to 1:4000 for both ELISA and immunoblot testing. User needs to try out the titration for immunohistochemical testing. To minimize background staining, we suggest using higher content of detergent (Tween-20) in dilution the Abs and washing.
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID5664
  • Target name
    PSEN2
  • Target description
    presenilin 2
  • Target synonyms
    AD3L; AD3LP; AD4; AD5; Alzheimer disease 4; CMD1V; E5-1; presenilin-2; PS2; PS-2; STM2; STM-2
  • Host
    Chicken
  • Isotype
    IgY
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Presenilin 2/AD5/PSEN2 using anti-Presenilin 2/AD5/PSEN2 antibody (A00800). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human CACO-2 whole cell lysates, Lane 4: mouse kidney tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Presenilin 2/AD5/PSEN2 antigen affinity purified polyclonal antibody (Catalog # A00800) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Presenilin 2/AD5/PSEN2 at approximately 34KD,50KD. The expected band size for Presenilin 2/AD5/PSEN2 is at 50KD.
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