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ProductsBack/PSMA1 Recombinant Antibody
Lane 1: Jurkat Cells; Lane 2: Hela Cells; Lane 3: HepG2 Cells; Lane 4: 293 Cells; Probed with PSMA1 (10A2) Monoclonal Antibody (bsm-54170R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Lane 1: Jurkat Cells; Lane 2: Hela Cells; Lane 3: HepG2 Cells; Lane 4: 293 Cells; Probed with PSMA1 (10A2) Monoclonal Antibody (bsm-54170R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Lane 1: Jurkat Cells; Lane 2: Hela Cells; Lane 3: HepG2 Cells; Lane 4: 293 Cells; Probed with PSMA1 (10A2) Monoclonal Antibody (bsm-54170R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.

PSMA1 Recombinant Antibody

BSM-54170R
Bioss Antibodies
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetPSMA1
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    PSMA1 (10A2) Monoclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(WB=1:500-2000), IHC-P(IHC-P=1:50-200), IHC-F(IHC-F=1:50-200), IF(IF=1:50-200), Flow-Cyt(Flow-Cyt=1:50), ICC/IF(ICC/IF=1:50)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID5682
  • Target name
    PSMA1
  • Target description
    proteasome 20S subunit alpha 1
  • Target synonyms
    30 kDa prosomal protein; epididymis secretory protein Li 275; HC2; HEL-S-275; macropain subunit C2; macropain subunit nu; multicatalytic endopeptidase complex subunit C2; NU; PROS30; PROS-30; proteasome (prosome, macropain) subunit, alpha type, 1; proteasome component C2; proteasome nu chain; proteasome subunit alpha 1; proteasome subunit alpha 6; proteasome subunit alpha type-1; proteasome subunit nu; proteasome subunit, alpha-type, 1; protein P30-33K; testicular tissue protein Li 150
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP25786
  • Protein Name
    Proteasome subunit alpha type-1
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

Datasheet

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Figure 1. Western blot analysis of PSMA1 using anti-PSMA1 antibody (A07591-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat liver tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PSMA1 antigen affinity purified polyclonal antibody (Catalog # A07591-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PSMA1 at approximately 33 kDa. The expected band size for PSMA1 is at 29-33 kDa.
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