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PSMB4 Antibody Pair

CSB-EAP01374
Cusabio
ApplicationsELISA
Product group Antibodies
ReactivityChicken, Guinea Pig, Goat, Human, Porcine
TargetPSMB4
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Overview

  • Supplier
    Cusabio
  • Product Name
    PSMB4 Antibody Pair
  • Delivery Days Customer
    9
  • Application Supplier Note
    We recommend using the capture antibody at a concentration of 0.2ug/ml and the detection antibody at a concentration of 0.25ug/ml.Optimal dilutions should be determined experimentally by the researcher.
  • Applications
    ELISA
  • Certification
    Research Use Only
  • Conjugate
    Biotin
  • Gene ID5692
  • Target name
    PSMB4
  • Target description
    proteasome 20S subunit beta 4
  • Target synonyms
    26 kDa prosomal protein; HN3; hsBPROS26; HsN3; macropain beta chain; multicatalytic endopeptidase complex beta chain; PRAAS3; PROS26; PROS-26; proteasome (prosome, macropain) subunit, beta type, 4; proteasome beta chain; proteasome chain 3; proteasome subunit beta 4; proteasome subunit beta type-4; proteasome subunit beta7; proteasome subunit HsN3; proteasome subunit, beta type, 4; testis tissue sperm-binding protein Li 79P
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP28070
  • Protein Name
    Proteasome subunit beta type-4
  • Reactivity
    Chicken, Guinea Pig, Goat, Human, Porcine
  • UNSPSC
    41116163

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Figure 1. Western blot analysis of PSMB4 using anti-PSMB4 antibody (A05105-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: human RT4 whole cell lysates, Lane 5: human U251 whole cell lysates, Lane 6: human placenta tissue lysates, Lane 7: human MCF-7 whole cell lysates, Lane 8: rat skeletal muscle tissue lysates, Lane 9: mouse liver tissue lysates, Lane 10: mouse skeletal muscle tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PSMB4 antigen affinity purified polyclonal antibody (Catalog # A05105-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PSMB4 at approximately 25,29 kDa. The expected band size for PSMB4 is at 29 kDa.
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