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ProductsBack/PSMB4 (H-3) Alexa Fluor(r) 680

PSMB4 (H-3) Alexa Fluor(r) 680

SC-390878AF680
Santa Cruz Biotechnology
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetPSMB4
200 ug
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Overview

  • Supplier
    Santa Cruz Biotechnology
  • Product Name
    PSMB4 (H-3) Alexa Fluor(r) 680
  • Delivery Days Customer
    5
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot
  • Certification
    Research Use Only
  • Conjugate
    AlexaFluor 680
  • Gene ID5692
  • Target name
    PSMB4
  • Target description
    proteasome 20S subunit beta 4
  • Target synonyms
    HN3, HsN3, PRAAS3, PROS-26, PROS26, proteasome subunit beta type-4, 26 kDa prosomal protein, beta-7, hsBPROS26, macropain beta chain, multicatalytic endopeptidase complex beta chain, proteasome (prosome, macropain) subunit, beta type, 4, proteasome beta chain, proteasome chain 3, proteasome subunit HsN3, proteasome subunit beta 4, proteasome subunit beta-7, proteasome subunit beta7, proteasome subunit, beta type, 4, testis tissue sperm-binding protein Li 79P
  • Host
    Mouse
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PSMB4 using anti-PSMB4 antibody (A05105-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: human RT4 whole cell lysates, Lane 5: human U251 whole cell lysates, Lane 6: human placenta tissue lysates, Lane 7: human MCF-7 whole cell lysates, Lane 8: rat skeletal muscle tissue lysates, Lane 9: mouse liver tissue lysates, Lane 10: mouse skeletal muscle tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PSMB4 antigen affinity purified polyclonal antibody (Catalog # A05105-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PSMB4 at approximately 25,29 kDa. The expected band size for PSMB4 is at 29 kDa.
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