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Western Blot Positive WB detected in: Mouse lung tissue All lanes: PSMB5 antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 29, 22, 18 kDa Observed band size: 29 kDa
Western Blot Positive WB detected in: Mouse lung tissue All lanes: PSMB5 antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 29, 22, 18 kDa Observed band size: 29 kDa
Western Blot Positive WB detected in: Mouse lung tissue All lanes: PSMB5 antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 29, 22, 18 kDa Observed band size: 29 kDa

PSMB5 Antibody

CSB-PA010083LA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetPSMB5
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Overview

  • Supplier
    Cusabio
  • Product Name
    PSMB5 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5693
  • Target name
    PSMB5
  • Target description
    proteasome 20S subunit beta 5
  • Target synonyms
    LMPX; macropain epsilon chain; MB1; multicatalytic endopeptidase complex epsilon chain; proteasome (prosome, macropain) subunit, beta type, 5; proteasome beta 5 subunit; proteasome catalytic subunit 3; proteasome chain 6; proteasome epsilon chain; proteasome subunit beta 5; proteasome subunit beta type-5; proteasome subunit MB1; proteasome subunit X; proteasome subunit, beta type, 5; PSX large multifunctional protease X; testicular tissue protein Li 153; X
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP28074
  • Protein Name
    Proteasome subunit beta type-5
  • Scientific Description
    The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This unit is responsible of the chymotrypsin-like activity of the proteasome and is one of the principal target of the proteasome inhibitor bortezomib. May catalyze basal processing of intracellular antigens. Plays a role in the protection against oxidative damage through the Nrf2-ARE pathway (By similarity).
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PSMB5/MB1 using anti-PSMB5/MB1 antibody (A03418-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: rat liver tissue lysates, Lane 5: rat pancreas tissue lysates, Lane 6: rat testis tissue lysates, Lane 7: rat kidney tissue lysates, Lane 8: mouse liver tissue lysates, Lane 9: mouse testis tissue lysates, Lane 10: mouse kidney tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PSMB5/MB1 antigen affinity purified polyclonal antibody (Catalog # A03418-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PSMB5/MB1 at approximately 22KD. The expected band size for PSMB5/MB1 is at 22KD.
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