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ProductsBack/PSMB9 Antibody (clone OTI1G7)

PSMB9 Antibody (clone OTI1G7)

LS-C173774
LifeSpan BioSciences
ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetPSMB9
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    PSMB9 Antibody (clone OTI1G7)
  • Delivery Days Customer
    23
  • Applications
    Flow Cytometry, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    Flo (1:100), IHC, IHC-P (1:150), WB (1:2000)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI1G7
  • Concentration
    0.77 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID5698
  • Target name
    PSMB9
  • Target description
    proteasome 20S subunit beta 9
  • Target synonyms
    beta1i; large multifunctional peptidase 2; LMP2; low molecular mass protein 2; macropain chain 7; multicatalytic endopeptidase complex chain 7; PRAAS3; proteasome (prosome, macropain) subunit, beta type, 9 (large multifunctional peptidase 2); proteasome catalytic subunit 1i; proteasome chain 7; proteasome subunit beta 6i; proteasome subunit beta 9; proteasome subunit beta type-9; proteasome subunit beta1i; proteasome subunit beta-1i; proteasome-related gene 2; PSMB6i; really interesting new gene 12 protein; RING12
  • Host
    Mouse
  • Isotype
    IgG1
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PSMB9 using anti-PSMB9 antibody (A02867-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human THP-1 whole cell lysates, Lane 5: rat spleen tissue lysates, Lane 6: rat thymus tissue lysates, Lane 7: mouse spleen tissue lysates, Lane 8: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PSMB9 antigen affinity purified polyclonal antibody (Catalog # A02867-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PSMB9 at approximately 21 kDa. The expected band size for PSMB9 is at 23 kDa.
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Western Blot Positive WB detected in: Raji whole cell lysate, HepG2 whole cell lysate, K562 whole cell lysate All lanes: PSMB9 antibody at 10microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 24, 23 kDa Observed band size: 24 kDa
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WB analysis of whole cell extracts (30 microglysate) of HeLa (Lane 1), HeLa treated with IFN gamma (100ng/ml IFN gamma for 72h) (Lane 2), THP-1 (Lane 3), THP-1 treated with PMA (50 ng PMA for 48h) and LPS (200 ng/ml LPS for 24 h) (Lane 4), U-937 (Lane 5), Raji (Lane 6) and Ramos (Lane 7) using GTX23328 LMP2 antibody. Dilution : 2 microg/ml
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