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Western Blot Positive WB detected in: Raji whole cell lysate, HepG2 whole cell lysate, K562 whole cell lysate All lanes: PSMB9 antibody at 10microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 24, 23 kDa Observed band size: 24 kDa
Western Blot Positive WB detected in: Raji whole cell lysate, HepG2 whole cell lysate, K562 whole cell lysate All lanes: PSMB9 antibody at 10microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 24, 23 kDa Observed band size: 24 kDa
Western Blot Positive WB detected in: Raji whole cell lysate, HepG2 whole cell lysate, K562 whole cell lysate All lanes: PSMB9 antibody at 10microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 24, 23 kDa Observed band size: 24 kDa

PSMB9 Antibody

CSB-PA018887LA01HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetPSMB9
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Overview

  • Supplier
    Cusabio
  • Product Name
    PSMB9 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5698
  • Target name
    PSMB9
  • Target description
    proteasome 20S subunit beta 9
  • Target synonyms
    beta1i; large multifunctional peptidase 2; LMP2; low molecular mass protein 2; macropain chain 7; multicatalytic endopeptidase complex chain 7; PRAAS3; proteasome (prosome, macropain) subunit, beta type, 9 (large multifunctional peptidase 2); proteasome catalytic subunit 1i; proteasome chain 7; proteasome subunit beta 6i; proteasome subunit beta 9; proteasome subunit beta type-9; proteasome subunit beta1i; proteasome subunit beta-1i; proteasome-related gene 2; PSMB6i; really interesting new gene 12 protein; RING12
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP28065
  • Protein Name
    Proteasome subunit beta type-9
  • Scientific Description
    The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit is involved in antigen processing to generate class I binding peptides. Replacement of PSMB6 by PSMB9 increases the capacity of the immunoproteasome to cleave model peptides after hydrophobic and basic residues.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PSMB9 using anti-PSMB9 antibody (A02867-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human THP-1 whole cell lysates, Lane 5: rat spleen tissue lysates, Lane 6: rat thymus tissue lysates, Lane 7: mouse spleen tissue lysates, Lane 8: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PSMB9 antigen affinity purified polyclonal antibody (Catalog # A02867-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PSMB9 at approximately 21 kDa. The expected band size for PSMB9 is at 23 kDa.
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