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PSMC5 Rabbit pAb

A1538
ABclonal
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
Product group Antibodies
TargetPSMC5
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Overview

  • Supplier
    ABclonal
  • Product Name
    PSMC5 Rabbit pAb
  • Delivery Days Customer
    12
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
  • Applications Supplier
    WB,IF/ICC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5705
  • Target name
    PSMC5
  • Target description
    proteasome 26S subunit, ATPase 5
  • Target synonyms
    RPT6, S8, SUG-1, SUG1, TBP10, TRIP1, p45, p45/SUG, 26S proteasome regulatory subunit 8, 26S protease regulatory subunit 8, 26S proteasome AAA-ATPase subunit RPT6, MSUG1 protein, Tat-binding protein homolog 10, proteasome (prosome, macropain) 26S subunit, ATPase, 5, proteasome 26S ATPase subunit 5, proteasome subunit p45, testicular tissue protein Li 149, thyroid hormone receptor-interacting protein 1, thyroid receptor interactor 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP62195
  • Protein Name
    26S proteasome regulatory subunit 8
  • Reactivity Supplier Note
    Human,Mouse,Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PSMC5 using anti-PSMC5 antibody (A05480-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human CCRF-CEM whole cell lysates, Lane 2: human MOLT-4 whole cell lysates, Lane 3: human SK-OV-3 whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: rat kidney tissue lysates, Lane 6: rat PC-12 whole cell lysates, Lane 7: mouse kidney tissue lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PSMC5 antigen affinity purified polyclonal antibody (Catalog # A05480-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PSMC5 at approximately 45-49 kDa. The expected band size for PSMC5 is at 45-49 kDa.
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