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Western blot All lanes: PSMD13antibody at 3.42ug/ml Lane 1: Mouse lung tissue Lane 2: 293T whole cell lysate Lane 3: Hela whole cell lysate Lane 4: HepG2 whole cell lysate Lane 5: Jurkat whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 43 kDa Observed band size: 43 kDa
Western blot All lanes: PSMD13antibody at 3.42ug/ml Lane 1: Mouse lung tissue Lane 2: 293T whole cell lysate Lane 3: Hela whole cell lysate Lane 4: HepG2 whole cell lysate Lane 5: Jurkat whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 43 kDa Observed band size: 43 kDa
Western blot All lanes: PSMD13antibody at 3.42ug/ml Lane 1: Mouse lung tissue Lane 2: 293T whole cell lysate Lane 3: Hela whole cell lysate Lane 4: HepG2 whole cell lysate Lane 5: Jurkat whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 43 kDa Observed band size: 43 kDa

PSMD13 Antibody

CSB-PA890768ESR1HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetPSMD13
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Overview

  • Supplier
    Cusabio
  • Product Name
    PSMD13 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5719
  • Target name
    PSMD13
  • Target description
    proteasome 26S subunit, non-ATPase 13
  • Target synonyms
    26S proteasome non-ATPase regulatory subunit 13; 26S proteasome regulatory subunit p40.5; 26S proteasome regulatory subunit RPN9; 26S proteasome regulatory subunit S11; 26S proteasome subunit p40.5; HSPC027; p40.5; proteasome (prosome, macropain) 26S subunit, non-ATPase, 13; Rpn9; S11
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UNM6
  • Protein Name
    26S proteasome non-ATPase regulatory subunit 13
  • Scientific Description
    Acts as a regulatory subunit of the 26S proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of PSMD13 using anti-PSMD13 antibody (A09882-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: rat lung tissue lysates, Lane 6: rat brain tissue lysates, Lane 7: mouse lung tissue lysates, Lane 8: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PSMD13 antigen affinity purified polyclonal antibody (Catalog # A09882-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PSMD13 at approximately 43 kDa. The expected band size for PSMD13 is at 43 kDa.
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