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Western Blot Positive WB detected in: 293 whole cell lysate All lanes: PSMD7 antibody at 3.2ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 38 kDa Observed band size: 38 kDa
Western Blot Positive WB detected in: 293 whole cell lysate All lanes: PSMD7 antibody at 3.2ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 38 kDa Observed band size: 38 kDa
Western Blot Positive WB detected in: 293 whole cell lysate All lanes: PSMD7 antibody at 3.2ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 38 kDa Observed band size: 38 kDa

PSMD7 Antibody

CSB-PA018912LA01HU
Cusabio
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetPSMD7
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Overview

  • Supplier
    Cusabio
  • Product Name
    PSMD7 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5713
  • Target name
    PSMD7
  • Target description
    proteasome 26S subunit, non-ATPase 7
  • Target synonyms
    26S proteasome non-ATPase regulatory subunit 7; 26S proteasome regulatory subunit rpn8; 26S proteasome regulatory subunit S12; Moloney leukemia virus-34 proviral integration; MOV34; Mov34 homolog; P40; proteasome (prosome, macropain) 26S subunit, non-ATPase, 7 (Mov34 homolog); proteasome subunit p40; Rpn8; S12
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP51665
  • Protein Name
    26S proteasome non-ATPase regulatory subunit 7
  • Scientific Description
    Acts as a regulatory subunit of the 26S proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PSMD7 using anti-PSMD7 antibody (A09527-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human Caco-2 whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: rat RH35 whole cell lysates, Lane 7: mouse liver tissue lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PSMD7 antigen affinity purified polyclonal antibody (Catalog # A09527-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PSMD7 at approximately 38 kDa. The expected band size for PSMD7 is at 37 kDa.
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