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Western blot All lanes: PSMF1 antibody at 1.28ug/ml Lane 1: A549 whole cell lysate Lane 2: K562 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 30 kDa Observed band size: 30 kDa
Western blot All lanes: PSMF1 antibody at 1.28ug/ml Lane 1: A549 whole cell lysate Lane 2: K562 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 30 kDa Observed band size: 30 kDa
Western blot All lanes: PSMF1 antibody at 1.28ug/ml Lane 1: A549 whole cell lysate Lane 2: K562 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 30 kDa Observed band size: 30 kDa

PSMF1 Antibody

CSB-PA852882DSR1HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetPSMF1
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Overview

  • Supplier
    Cusabio
  • Product Name
    PSMF1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID9491
  • Target name
    PSMF1
  • Target description
    proteasome inhibitor subunit 1
  • Target synonyms
    PI31, proteasome inhibitor PI31 subunit, proteasome (prosome, macropain) inhibitor subunit 1 (PI31)
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ92530
  • Protein Name
    Proteasome inhibitor PI31 subunit
  • Scientific Description
    Plays an important role in control of proteasome function. Inhibits the hydrolysis of protein and peptide substrates by the 20S proteasome. Also inhibits the activation of the proteasome by the proteasome regulatory proteins PA700 and PA28.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PSMF1 using anti-PSMF1 antibody (A08650-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PSMF1 antigen affinity purified polyclonal antibody (Catalog # A08650-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PSMF1 at approximately 30 kDa. The expected band size for PSMF1 is at 30 kDa.
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