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ProductsBack/Psoriasin antibody [47C1068]
ICC/IF analysis of (A) untreated or (B) LPS-treated (100 ng/ml, 21 hrs) HOPE-fixed NCI-H727 cells using GTX13680 Psoriasin antibody [47C1068]. Positive control(C) is shown by the expression of the nuclear Ki67 antigen with the MIB-1 (2 ug/ml) antibody. Negative control(D) was included omitting the primary antibody.
ICC/IF analysis of (A) untreated or (B) LPS-treated (100 ng/ml, 21 hrs) HOPE-fixed NCI-H727 cells using GTX13680 Psoriasin antibody [47C1068]. Positive control(C) is shown by the expression of the nuclear Ki67 antigen with the MIB-1 (2 ug/ml) antibody. Negative control(D) was included omitting the primary antibody.
ICC/IF analysis of (A) untreated or (B) LPS-treated (100 ng/ml, 21 hrs) HOPE-fixed NCI-H727 cells using GTX13680 Psoriasin antibody [47C1068]. Positive control(C) is shown by the expression of the nuclear Ki67 antigen with the MIB-1 (2 ug/ml) antibody. Negative control(D) was included omitting the primary antibody.

Psoriasin antibody [47C1068]

GTX13680
GeneTex
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetS100A7
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Overview

  • Supplier
    GeneTex
  • Product Name
    Psoriasin antibody [47C1068]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1 - 3 microg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    47C1068
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID6278
  • Target name
    S100A7
  • Target description
    S100 calcium binding protein A7
  • Target synonyms
    PSOR1, S100A7c, protein S100-A7, psoriasin 1
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP31151
  • Protein Name
    Protein S100-A7
  • Scientific Description
    The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein differs from the other S100 proteins of known structure in its lack of calcium binding ability in one EF-hand at the N-terminus. This protein is markedly over-expressed in the skin lesions of psoriatic patients, but is excluded as a candidate gene for familial psoriasis susceptibility. The exact function of this protein is not known. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Psoriasin using anti-Psoriasin antibody (A02369-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human Hela cell lysates, Lane 3: rat spleen tissue lysates, Lane 4: rat RH35 cell lysates, Lane 5: mouse spleen tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Psoriasin antigen affinity purified polyclonal antibody (Catalog # A02369-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Psoriasin at approximately 17KD. The expected band size for Psoriasin is at 11KD.
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