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PSPC1 Antibody

CSB-PA018937LA01HU
Cusabio
ApplicationsELISA
Product group Antibodies
ReactivityHuman
TargetPSPC1
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Overview

  • Supplier
    Cusabio
  • Product Name
    PSPC1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID55269
  • Target name
    PSPC1
  • Target description
    paraspeckle component 1
  • Target synonyms
    PSP1, paraspeckle component 1, paraspeckle protein 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8WXF1
  • Protein Name
    Paraspeckle component 1
  • Scientific Description
    Regulates, cooperatively with NONO and SFPQ, androgen receptor-mediated gene transcription activity in Sertoli cell line (By similarity). Binds to poly(A), poly(G) and poly(U) RNA homopolymers. Regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-ARNTL/BMAL1 heterodimer (By similarity). Together with NONO, required for the formation of nuclear paraspeckles.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PSPC1 using anti-PSPC1 antibody (A06348-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human U20S whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PSPC1 antigen affinity purified polyclonal antibody (Catalog # A06348-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PSPC1 at approximately 66 kDa. The expected band size for PSPC1 is at 66 kDa.
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