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PSTPIP1 Antibody

LS-C409311
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
TargetPSTPIP1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    PSTPIP1 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 45kDa/47kDa, while the observed MW by Western blot was 48kDa.
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    IHC (1:50 - 1:200), WB (1:500 - 1:2000) The predicted MW is 45kDa/47kDa, while the observed MW by Western blot was 48kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.85 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID9051
  • Target name
    PSTPIP1
  • Target description
    proline-serine-threonine phosphatase interacting protein 1
  • Target synonyms
    AICZC, CD2BP1, CD2BP1L, CD2BP1S, H-PIP, PAPA, PAPAS, PSTPIP, proline-serine-threonine phosphatase-interacting protein 1, CD2 antigen-binding protein 1, CD2 cytoplasmic tail-binding protein, CD2-binding protein 1, PEST phosphatase-interacting protein 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PSTPIP1 using anti-PSTPIP1 antibody (A04844-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PSTPIP1 antigen affinity purified polyclonal antibody (Catalog # A04844-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PSTPIP1 at approximately 45 kDa. The expected band size for PSTPIP1 is at 48 kDa.
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