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Western blot All lanes: PSTPIP2 antibody at 6ug/ml + Raji whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 39, 37 kDa Observed band size: 39 kDa
Western blot All lanes: PSTPIP2 antibody at 6ug/ml + Raji whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 39, 37 kDa Observed band size: 39 kDa
Western blot All lanes: PSTPIP2 antibody at 6ug/ml + Raji whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 39, 37 kDa Observed band size: 39 kDa

PSTPIP2 Antibody

CSB-PA884494LA01HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetPSTPIP2
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Overview

  • Supplier
    Cusabio
  • Product Name
    PSTPIP2 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID9050
  • Target name
    PSTPIP2
  • Target description
    proline-serine-threonine phosphatase interacting protein 2
  • Target synonyms
    MAYP, proline-serine-threonine phosphatase-interacting protein 2, PEST phosphatase-interacting protein 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9H939
  • Protein Name
    Proline-serine-threonine phosphatase-interacting protein 2
  • Scientific Description
    Binds to F-actin. May be involved in regulation of the actin cytoskeleton (By similarity).
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PSTPIP2 using anti-PSTPIP2 antibody (A16132-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PSTPIP2 antigen affinity purified polyclonal antibody (Catalog # A16132-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PSTPIP2 at approximately 36,39 kDa. The expected band size for PSTPIP2 is at 36,39 kDa.
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Immunohistochemistry analysis in human spleen and pancreas tissues using HPA040944 antibody. Corresponding PSTPIP2 RNA-seq data are presented for the same tissues.
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