More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/PTCH2 / Patched 2 Antibody (aa362-411)

PTCH2 / Patched 2 Antibody (aa362-411)

LS-B11475
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetPTCH2
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    PTCH2 / Patched 2 Antibody (aa362-411)
  • Delivery Days Customer
    14
  • Application Supplier Note
    ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IHC, IHC-P (10 µg/ml), WB ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID8643
  • Target name
    PTCH2
  • Target description
    patched 2
  • Target synonyms
    PTC2, SLC65B2, protein patched homolog 2, patched homolog 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

FAQ

Go to our frequently asked questions

Related products

Figure 1. Western blot analysis of PTCH2 using anti-PTCH2 antibody (A06375-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTCH2 antigen affinity purified polyclonal antibody (Catalog # A06375-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PTCH2 at approximately 150KD. The expected band size for PTCH2 is at 150KD.
Product group Antibodies
Boster Bio
Anti-PTCH2 Antibody Picoband(r)A06375-1-CARRIER-FREE
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetPTCH2
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
PTCH2 / Patched 2 AntibodyLS-C673600
ApplicationsELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman
TargetPTCH2
  • SizePrice
Product group Antibodies
Bioss
Anti-PTCH2 AntibodyBS-70825R
ApplicationsWestern Blot
ReactivityHuman, Mouse
TargetPTCH2
  • SizePrice
IHC image of CSB-PA896928LA01HU diluted at 1:200 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Product group Antibodies
Cusabio
PTCH2 AntibodyCSB-PA896928LA01HU
ApplicationsImmunoFluorescence, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetPTCH2
  • SizePrice
WB analysis of human placenta tissue using GTX46542 PTCH2 antibody at 0.2-1μg/ml.
Product group Antibodies
GeneTex
PTCH2 antibody, N-termGTX46542
ApplicationsWestern Blot
ReactivityHuman
TargetPTCH2
  • SizePrice