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Western Blot analysis of K562 cells using p23 Polyclonal Antibody
Western Blot analysis of K562 cells using p23 Polyclonal Antibody
Western Blot analysis of K562 cells using p23 Polyclonal Antibody

PTGES3 Antibody

CSB-PA003624
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetPTGES3
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Overview

  • Supplier
    Cusabio
  • Product Name
    PTGES3 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10728
  • Target name
    PTGES3
  • Target description
    prostaglandin E synthase 3
  • Target synonyms
    cPGES; cytosolic prostaglandin E synthase; cytosolic prostaglandin E2 synthase; Hsp90 co-chaperone; P23; progesterone receptor complex p23; prostaglandin E synthase 3; prostaglandin E synthase 3 (cytosolic); TEBP; telomerase-binding protein p23; unactive progesterone receptor, 23 kD
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ15185
  • Protein Name
    Prostaglandin E synthase 3
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PTGES3 using anti-PTGES3 antibody (A04136-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HELA whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human HEPG2 whole cell lysates, Lane 4: human COLO-320 whole cell lysates, Lane 5: human Raji whole cell lysates, Lane 6: human HEK293 whole cell lysates, Lane 7: human K562 whole cell lysates, Lane 8: human THP-1 whole cell lysates, Lane 9: rat PC-12 whole cell lysates, Lane 10: mouse NIH/3T3 whole cell lysates, Lane 11: mouse HEPA1-6 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTGES3 antigen affinity purified polyclonal antibody (Catalog # A04136-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PTGES3 at approximately 23KD. The expected band size for PTGES3 is at 23KD.
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