Non-transfected (–) and transfected (+) boiled and unboiled 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PTH1R antibody [HL3651] (GTX641642) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
![Unboiled mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with PTH1R antibody [HL3651] (GTX641642) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX641642/GTX641642_T-45642_20250110_WB_M_kidney_25011618_801.webp "Unboiled mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with PTH1R antibody [HL3651] (GTX641642) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![PTH1R antibody [HL3651] detects PTH1R protein by immunohistochemical analysis. Sample: Frozen-sectioned mouse kidney. Green: PTH1R stained by PTH1R antibody [HL3651] (GTX641642) diluted at 1:250. Red: Vimentin antibody [GT812] (GTX629744) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920).](https://www.genetex.com/upload/website/prouct_img/normal/GTX641642/GTX641642_T-45642_20250415_IHC-Fr_M_25042420_440.webp "PTH1R antibody [HL3651] detects PTH1R protein by immunohistochemical analysis. Sample: Frozen-sectioned mouse kidney. Green: PTH1R stained by PTH1R antibody [HL3651] (GTX641642) diluted at 1:250. Red: Vimentin antibody [GT812] (GTX629744) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920).")
![PTH1R antibody [HL3651] (GTX641642) detects PTH1R protein by flow cytometry analysis. Sample: Non-transfected and transfected 293T cells were fixed in 4% paraformaldehyde at 4oC for 15 min and washed with 0.1% saponin buffer. The cells were following stained with PTH1R antibody [HL3651] (GTX641642) diluted at 1:50 or a Rabbit IgG isotype control (GTX35035) at 4oC for 1 hour.](https://www.genetex.com/upload/website/prouct_img/normal/GTX641642/GTX641642_45712_20250919_FCM_25100122_966.webp "PTH1R antibody [HL3651] (GTX641642) detects PTH1R protein by flow cytometry analysis. Sample: Non-transfected and transfected 293T cells were fixed in 4% paraformaldehyde at 4oC for 15 min and washed with 0.1% saponin buffer. The cells were following stained with PTH1R antibody [HL3651] (GTX641642) diluted at 1:50 or a Rabbit IgG isotype control (GTX35035) at 4oC for 1 hour.")
Non-transfected (–) and transfected (+) boiled and unboiled 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PTH1R antibody [HL3651] (GTX641642) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
PTH1R antibody [HL3651]
GTX641642
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse
TargetPTH1R
Overview
- SupplierGeneTex
- Product NamePTH1R antibody [HL3651]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL3651
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID5745
- Target namePTH1R
- Target descriptionparathyroid hormone 1 receptor
- Target synonymsEKNS, PFE, PTHR, PTHR1, parathyroid hormone/parathyroid hormone-related peptide receptor, PTH/PTHr receptor, PTH/PTHrP type I receptor, PTH1 receptor, parathyroid hormone receptor 1, parathyroid hormone/parathyroid hormone-related protein receptor, seven transmembrane helix receptor
- HostRabbit
- IsotypeIgG
- Protein IDQ03431
- Protein NameParathyroid hormone/parathyroid hormone-related peptide receptor
- Scientific DescriptionThe protein encoded by this gene is a member of the G-protein coupled receptor family 2. This protein is a receptor for parathyroid hormone (PTH) and for parathyroid hormone-like hormone (PTHLH). The activity of this receptor is mediated by G proteins which activate adenylyl cyclase and also a phosphatidylinositol-calcium second messenger system. Defects in this receptor are known to be the cause of Jansens metaphyseal chondrodysplasia (JMC), chondrodysplasia Blomstrand type (BOCD), as well as enchodromatosis. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, May 2010]
- ReactivityHuman, Mouse
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
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