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WB analysis of various sample lysates using GTX54674 PTPIP51 antibody. Dilution : 1:1000 Loading : 25μg per lane
WB analysis of various sample lysates using GTX54674 PTPIP51 antibody. Dilution : 1:1000 Loading : 25μg per lane
WB analysis of various sample lysates using GTX54674 PTPIP51 antibody. Dilution : 1:1000 Loading : 25μg per lane

PTPIP51 antibody

GTX54674
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetRMDN3
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Overview

  • Supplier
    GeneTex
  • Product Name
    PTPIP51 antibody
  • Delivery Days Customer
    7
  • Application Supplier Note
    WB: 1:500 - 1:2000. IHC-P: 1:50 - 1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID55177
  • Target name
    RMDN3
  • Target description
    regulator of microtubule dynamics 3
  • Target synonyms
    FAM82A2, FAM82C, RMD-3, RMD3, ptpip51, regulator of microtubule dynamics protein 3, TCPTP-interacting protein 51, cerebral protein 10, family with sequence similarity 82, member A2, family with sequence similarity 82, member C, microtubule-associated protein, protein tyrosine phosphatase-interacting protein 51
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96TC7
  • Protein Name
    Regulator of microtubule dynamics protein 3
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    41116161

References

  • alpha-Synuclein binds to the ER-mitochondria tethering protein VAPB to disrupt Ca2+ homeostasis and mitochondrial ATP production. Paillusson S et al., 2017 Jul, Acta Neuropathol
    Read this paper
  • ER-mitochondria contacts control surface glycan expression and sensitivity to killer lymphocytes in glioma stem-like cells. Bassoy EY et al., 2017 Jun 1, EMBO J
    Read this paper

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Figure 1. Western blot analysis of PTPIP51/RMDN3 using anti-PTPIP51/RMDN3 antibody (A09297). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hacat whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTPIP51/RMDN3 antigen affinity purified polyclonal antibody (Catalog # A09297) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PTPIP51/RMDN3 at approximately 55 kDa. The expected band size for PTPIP51/RMDN3 is at 52 kDa.
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