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Western Blot analysis of COS7 cells using PTP1B Polyclonal Antibody
Western Blot analysis of COS7 cells using PTP1B Polyclonal Antibody
Western Blot analysis of COS7 cells using PTP1B Polyclonal Antibody

PTPN1 Antibody

CSB-PA003883
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Monkey, Mouse, Rat
TargetPTPN1
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Overview

  • Supplier
    Cusabio
  • Product Name
    PTPN1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5770
  • Target name
    PTPN1
  • Target description
    protein tyrosine phosphatase non-receptor type 1
  • Target synonyms
    protein tyrosine phosphatase, placental; protein-tyrosine phosphatase 1B; PTP1B; tyrosine-protein phosphatase non-receptor type 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP18031
  • Protein Name
    Tyrosine-protein phosphatase non-receptor type 1
  • Reactivity
    Human, Monkey, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PTP1B using anti-PTP1B antibody (A00613-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat kidney tissue lysate, Lane 2: mouse kidney tissue lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTP1B antigen affinity purified polyclonal antibody (Catalog # A00613-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PTP1B at approximately 57KD. The expected band size for PTP1B is at 50KD.
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