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Western Blot Positive WB detected in: Hela whole cell lysate, NIH/3T3 whole cell lysate, Rat kidney tissue All lanes: PTPN12 antibody at 7.4microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 89, 73, 75 kDa Observed band size: 89 kDa
Western Blot Positive WB detected in: Hela whole cell lysate, NIH/3T3 whole cell lysate, Rat kidney tissue All lanes: PTPN12 antibody at 7.4microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 89, 73, 75 kDa Observed band size: 89 kDa
Western Blot Positive WB detected in: Hela whole cell lysate, NIH/3T3 whole cell lysate, Rat kidney tissue All lanes: PTPN12 antibody at 7.4microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 89, 73, 75 kDa Observed band size: 89 kDa

PTPN12 Antibody

CSB-PA019026LA01HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetPTPN12
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Overview

  • Supplier
    Cusabio
  • Product Name
    PTPN12 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5782
  • Target name
    PTPN12
  • Target description
    protein tyrosine phosphatase non-receptor type 12
  • Target synonyms
    protein-tyrosine phosphatase G1; PTPG1; PTP-PEST; tyrosine-protein phosphatase non-receptor type 12
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ05209
  • Protein Name
    Tyrosine-protein phosphatase non-receptor type 12
  • Scientific Description
    Dephosphorylates a range of proteins, and thereby regulates cellular signaling cascades (PubMed:18559503). Dephosphorylates cellular tyrosine kinases, such as ERBB2 and PTK2B/PYK2, and thereby regulates signaling via ERBB2 and PTK2B/PYK2 (PubMed:17329398, PubMed:27134172). Selectively dephosphorylates ERBB2 phosphorylated at Tyr-1112, Tyr-1196, and/or Tyr-1248 (PubMed:27134172).
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PTPN12 using anti-PTPN12 antibody (A02762-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HL-60 whole cell lysates, Lane 2: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTPN12 antigen affinity purified polyclonal antibody (Catalog # A02762-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PTPN12 at approximately 110 kDa. The expected band size for PTPN12 is at 88 kDa.
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