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Western Blot analysis of NIH-3T3 cells using SH-PTP1 Polyclonal Antibody
Western Blot analysis of NIH-3T3 cells using SH-PTP1 Polyclonal Antibody
Western Blot analysis of NIH-3T3 cells using SH-PTP1 Polyclonal Antibody

PTPN6 Antibody

CSB-PA004085
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetPTPN6
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Overview

  • Supplier
    Cusabio
  • Product Name
    PTPN6 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5777
  • Target name
    PTPN6
  • Target description
    protein tyrosine phosphatase non-receptor type 6
  • Target synonyms
    HCP, HCPH, HPTP1C, PTP-1C, SH-PTP1, SHP-1, SHP-1L, SHP1, tyrosine-protein phosphatase non-receptor type 6, hematopoietic cell phosphatase, hematopoietic cell protein-tyrosine phosphatase, protein-tyrosine phosphatase 1C, protein-tyrosine phosphatase SHP-1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP29350
  • Protein Name
    Tyrosine-protein phosphatase non-receptor type 6
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SHP1/PTPN6 using anti-SHP1/PTPN6 antibody (A00938-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human Raji whole cell lysates, Lane 3: mouse Raw264.7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SHP1/PTPN6 antigen affinity purified polyclonal antibody (Catalog # A00938-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SHP1/PTPN6 at approximately 68KD. The expected band size for SHP1/PTPN6 is at 68KD.
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