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Pulmonary Surfactant Associated Protein A1 (SFTPA1) Polyclonal Antibody

CAU26869
Biomatik
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
TargetSFTPA1
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Overview

  • Supplier
    Biomatik
  • Product Name
    Pulmonary Surfactant Associated Protein A1 (SFTPA1) Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    WB; IHC; ICC; IP.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID653509
  • Target name
    SFTPA1
  • Target description
    surfactant protein A1
  • Target synonyms
    35 kDa pulmonary surfactant-associated protein; alveolar proteinosis protein; COLEC4; collectin-4; PSAP; PSPA; PSP-A; pulmonary surfactant-associated protein A1; SFTP1; SFTPA1B; SPA; SP-A; SPA1; SP-A1; SP-A1 beta; SP-A1 delta; SP-A1 epsilon; SP-A1 gamma; surfactant protein A1B; surfactant, pulmonary-associated protein A1A; surfactant, pulmonary-associated protein A1B
  • Host
    Rabbit
  • Protein IDQ8IWL2
  • Protein Name
    Pulmonary surfactant-associated protein A1
  • Scientific Description
    The Pulmonary Surfactant Associated Protein A1 (SFTPA1) Polyclonal Antibody (Species: Human) has been validated for the following applications: WB, IHC.
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SFTPA1/2 using anti-SFTPA1/2 antibody (PB9390). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat lung tissue lysates, Lane 2: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SFTPA1/2 antigen affinity purified polyclonal antibody (Catalog # PB9390) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SFTPA1/2 at approximately 26-38 kDa. The expected band size for SFTPA1/2 is at 26 kDa.
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