PUREfrex 2.0

Cosmo Bio USA

PUREfrex 2.0

16

Research Use Only

PUREfrex® 2.0 reconstituted coupled transcription/translation system is a new and improved version of PUREfrex® 1.0 that debuted in 2011. By improving the purification process for components of PUREfrex® 2.0RNase, beta-galactosidase and LPS contamination has been reduced, the latter to around 0.1EU/uL of reaction mixture. This, together with a newly optimized composition has boosted protein yield 2-10 times compared to PUREfrex® 1.0. Further, all proteinaceous components of PUREfrex® 2.0 are free of fusion tags, allowing users the freedom to incorporate any chosen tag for protein purification/detection. For proteins whose functional conformation requires the assistance of molecular chaperones or the creation of disulfide bonds, we offer supplemental reagents that work in conjunction with PUREfrex® 2.0. Supplements - Molecular Chaperones These are supplements for PUREfrex® series reconstituted coupled transcription/translation systems to promote production and solubility of aggregation-prone proteins. DnaK Mix (#PF003-0.5-EX) GroE Mix (#PF004-0.5-EX) Supplements - Forming disulfide bonds These are supplements for PUREfrex® series reconstituted coupled transcription/translation systems to promote synthesis of functional proteins containing disulfide bonds. DsbC Set (#PF005-0.5-EX) PDI Set (#PF006-0.5-EX)

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