More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/Purified Anti-Mouse CD86 Antibody

Purified Anti-Mouse CD86 Antibody

RD20100F
Reddot Biotech
ApplicationsFlow Cytometry
Product group Antibodies
TargetCd86
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    Reddot Biotech
  • Product Name
    Purified Anti-Mouse CD86 Antibody
  • Delivery Days Customer
    5
  • Applications
    Flow Cytometry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    GL-1
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID12524
  • Target name
    Cd86
  • Target description
    CD86 antigen
  • Target synonyms
    activation B7-2 antigen; B7; B7.2; B70; B7-2; Cd28l; Cd28l2; CLS1; early T cell costimulatory molecule-1; ETC-1; Ly5; Ly-5; Ly58; Ly-58; MB7; MB7-2; T-lymphocyte activation antigen CD86; TS/A-2
  • Host
    Rat
  • Isotype
    IgG2a
  • Scientific Description
    CD86 is an 80 kD immunoglobulin superfamily member also known as B7-2, B70, and Ly-58. CD86 is expressed on activated B and T cells, macrophages, dendritic cells, and astrocytes. CD86, along with CD80, is a ligand of CD28 and CD152 (CTLA-4). CD86 is expressed earlier in the immune response than CD80. CD86 has also been shown to be involved in immunoglobulin class-switching and triggering of NK cell-mediated cytotoxicity. CD86 binds to CD28 to transduce co-stimulatory signals for T cell activation, proliferation, and cytokine production. CD86 can also bind to CD152, also known as CTLA-4, to deliver an inhibitory signal to T cells.
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

Related products

Product group Antibodies
Biomatik
Cluster Of Differentiation 86 (CD86) Polyclonal AntibodyCAU27020
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetCd86
  • SizePrice
Product group Antibodies
RayBiotech
Anti-Mouse CD86 Antibody, Purified135-00041
ApplicationsFlow Cytometry
TargetCd86
  • SizePrice
Product group Antibodies
GeneTex
CD86 antibody [MAB0827]GTX52415
ApplicationsFlow Cytometry, ImmunoPrecipitation, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, Neutralisation/Blocking
TargetCd86
  • SizePrice
Product group Antibodies
Bethyl Laboratories
Rabbit anti-CD86 Recombinant Monoclonal Antibody [BLR255L]A700-255
ApplicationsFlow Cytometry, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
TargetCd86
  • SizePrice
Product group Antibodies
Assay Genie
Purified Anti-Mouse CD86 Antibody [GL-1]AGEL0039
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, ImmunoHistoChemistry
TargetCd86
  • SizePrice
Figure 1. Western blot analysis of Cd86 using anti-Cd86 antibody (A00220-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat lung tissue lysates, Lane 2: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cd86 antigen affinity purified polyclonal antibody (Catalog # A00220-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cd86 at approximately 60-80 kDa. The expected band size for Cd86 is at 35 kDa.
Product group Antibodies
Boster Bio
Anti-Cd86 Antibody Picoband(r)A00220-4-CARRIER-FREE
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
TargetCd86
  • SizePrice
Product group Antibodies
MedChem Express
Rat Anti-Mouse CD86 Antibody (FITC)HY-P83710M
TargetCd86
  • SizePrice
Product group Antibodies
Absolute Antibody
Anti-CD86 [PO.3]Ab01029-1.32
ApplicationsFunctional Assay, Flow Cytometry, Western Blot, ImmunoHistoChemistry, Neutralisation/Blocking
TargetCd86
  • SizePrice