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PXK Rabbit pAb

A15458
ABclonal
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetPXK
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Overview

  • Supplier
    ABclonal
  • Product Name
    PXK Rabbit pAb
  • Delivery Days Customer
    12
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID54899
  • Target name
    PXK
  • Target description
    PX domain containing serine/threonine kinase like
  • Target synonyms
    MONAKA, SLOB, PX domain-containing protein kinase-like protein, PX ser/thr kinase v2, PX serine/threonine kinase, modulator of Na,K-ATPase long form
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ7Z7A4
  • Protein Name
    PX domain-containing protein kinase-like protein
  • Reactivity Supplier Note
    Human,Mouse,Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Western Blot Positive WB detected in: HEK293 whole cell lysate, A549 whole cell lysate, Mouse brain tissue, Mouse heart tissue, Rat brain tissue All lanes: PXK antibody at 2.7microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 65, 59, 39, 63, 50, 61, 56 kDa Observed band size: 65 kDa
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Figure 1. Western blot analysis of PXK using anti-PXK antibody (A07555-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: monkey COS-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PXK antigen affinity purified polyclonal antibody (Catalog # A07555-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PXK at approximately 65 kDa. The expected band size for PXK is at 65 kDa.
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