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Pycard Antibody

CSB-PA861664ZA01MO
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetPycard
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Overview

  • Supplier
    Cusabio
  • Product Name
    Pycard Antibody
  • Delivery Days Customer
    9
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID66824
  • Target name
    Pycard
  • Target description
    PYD and CARD domain containing
  • Target synonyms
    9130417A21Rik; A; apoptosis-associated speck-like protein containing a CARD; Asc; CARD5; masc; PYD and CARD domain-containing protein; TMS-; TMS-1; TNS1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9EPB4
  • Protein Name
    Apoptosis-associated speck-like protein containing a CARD
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TMS1/ASC/PYCARD using anti-TMS1/ASC/PYCARD antibody (A00362-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat lung tissue lysates, Lane 2: rat spleen tissue lysates, Lane 3: rat thymus tissue lysates, Lane 4: rat heart tissue lysates, Lane 5: mouse lung tissue lysates, Lane 6: mouse spleen tissue lysates, Lane 7: mouse thymus tissue lysates, Lane 8: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TMS1/ASC/PYCARD antigen affinity purified polyclonal antibody (Catalog # A00362-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TMS1/ASC/PYCARD at approximately 22-25 kDa. The expected band size for TMS1/ASC/PYCARD is at 21 kDa.
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