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Immunohistochemistry of paraffin-embedded human breast cancer using CSB-PA019115HA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human breast cancer using CSB-PA019115HA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human breast cancer using CSB-PA019115HA01HU at dilution of 1:100

PYCR1 Antibody

CSB-PA019115HA01HU
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetPYCR1
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Overview

  • Supplier
    Cusabio
  • Product Name
    PYCR1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5831
  • Target name
    PYCR1
  • Target description
    pyrroline-5-carboxylate reductase 1
  • Target synonyms
    ARCL2B; ARCL3B; P5C; P5C reductase 1; P5CR; PIG45; PP222; PRO3; proliferation-inducing protein 45; PYCR; pyrroline-5-carboxylate reductase 1, mitochondrial
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP32322
  • Protein Name
    Pyrroline-5-carboxylate reductase 1, mitochondrial
  • Scientific Description
    Housekeeping enzyme that catalyzes the last step in proline biosynthesis. Can utilize both NAD and NADP, but has higher affinity for NAD. Involved in the cellular response to oxidative stress.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neurons.
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Figure 1. Western blot analysis of PYCR1 using anti-PYCR1 antibody (A06018-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SW620 whole cell lysates, Lane 2: human PANC-1 whole cell lysates, Lane 3: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PYCR1 antigen affinity purified polyclonal antibody (Catalog # A06018-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PYCR1 at approximately 35 kDa. The expected band size for PYCR1 is at 33,35 kDa.
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