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PYCRL Antibody (clone OTI5F7)

LS-C172373
LifeSpan BioSciences
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot
Product group Antibodies
TargetPYCR3
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    PYCRL Antibody (clone OTI5F7)
  • Delivery Days Customer
    23
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot
  • Applications Supplier
    Flo (1:100), IF (1:100), WB (1:500)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI5F7
  • Concentration
    0.69 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID65263
  • Target name
    PYCR3
  • Target description
    pyrroline-5-carboxylate reductase 3
  • Target synonyms
    P5C reductase 3; P5CR 3; PYCRL; pyrroline-5-carboxylate reductase 3; pyrroline-5-carboxylate reductase-like
  • Host
    Mouse
  • Isotype
    IgG2a
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PYCRL/PYCR3 using anti-PYCRL/PYCR3 antibody (A32428-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: mouse kidney tissue lysates, Lane 6: mouse small intestine tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PYCRL/PYCR3 antigen affinity purified polyclonal antibody (A32428-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PYCRL/PYCR3 at approximately 29 kDa. The expected band size for PYCRL/PYCR3 is at 29 kDa.
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