WB analysis of pervanadate-treated primary chicken embryo fibroblasts plated on fibronectin, either mock-transfected (Lane 1) or transfected with a Pyk2 expression vector (Lane 2-6) using GTX24801 PYK2 (phospho Tyr881) antibody. The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody.
WB analysis of pervanadate-treated primary chicken embryo fibroblasts plated on fibronectin, either mock-transfected (Lane 1) or transfected with a Pyk2 expression vector (Lane 2-6) using GTX24801 PYK2 (phospho Tyr881) antibody. The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody.
PYK2 (phospho Tyr881) antibody
GTX24801
ApplicationsFlow Cytometry, ImmunoPrecipitation, Western Blot
Product group Antibodies
ReactivityChicken, Human, Mouse, Rat
TargetPTK2B
Overview
- SupplierGeneTex
- Product NamePYK2 (phospho Tyr881) antibody - Orthogonal Validated
- Delivery Days Customer9
- Antibody SpecificitySome cross-reactivity with Focal Adhesion Kinase phosphorylated at tyrosine 925 (the corresponding Grb 2 binding site) may be observed in cases where Focal Adhesion Kinase is overexpressed relative to PYK 2 in the same cell system.
- Application Supplier NoteFACS: 1:20. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsFlow Cytometry, ImmunoPrecipitation, Western Blot
- CertificationResearch Use Only
- ClonalityPolyclonal
- ConjugateUnconjugated
- Gene ID2185
- Target namePTK2B
- Target descriptionprotein tyrosine kinase 2 beta
- Target synonymsCADTK; CAKB; CAK-beta; calcium-dependent tyrosine kinase; calcium-regulated non-receptor proline-rich tyrosine kinase; cell adhesion kinase beta; FADK 2; FADK2; FAK2; focal adhesion kinase 2; PKB; proline-rich tyrosine kinase 2; protein kinase B; protein-tyrosine kinase 2-beta; PTK; PTK2B protein tyrosine kinase 2 beta; PYK2; RAFTK; related adhesion focal tyrosine kinase
- HostRabbit
- IsotypeIgG
- Protein IDQ14289
- Protein NameProtein-tyrosine kinase 2-beta
- Scientific DescriptionThis gene encodes a cytoplasmic protein tyrosine kinase which is involved in calcium-induced regulation of ion channels and activation of the map kinase signaling pathway. The encoded protein may represent an important signaling intermediate between neuropeptide-activated receptors or neurotransmitters that increase calcium flux and the downstream signals that regulate neuronal activity. The encoded protein undergoes rapid tyrosine phosphorylation and activation in response to increases in the intracellular calcium concentration, nicotinic acetylcholine receptor activation, membrane depolarization, or protein kinase C activation. This protein has been shown to bind CRK-associated substrate, nephrocystin, GTPase regulator associated with FAK, and the SH2 domain of GRB2. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Four transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
- ReactivityChicken, Human, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203