Pyruvate Assay Kit (Colorimetric/Fluorometric)

LifeSpan BioSciences

Pyruvate Assay Kit (Colorimetric/Fluorometric)

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For quantitative determination of pyruvate and evaluation of drug effects on its metabolism.

Spectrophotometry (fluorometric), Spectrophotometry (visible) For quantitative determination of pyruvate and evaluation of drug effects on its metabolism.

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Pyruvate is a key intermediate in cellular metabolic pathways. Pyruvate can be converted to carbohydrates via gluconeogenesis, to fatty acids or energy through acetyl-CoA, to the amino acid alanine and to ethanol. Abnormal levels of pyruvate have been linked to liver diseases and metabolic disorders. Simple, direct and automation-ready procedures for measuring pyruvate concentrations find wide applications in research and drug discovery. This pyruvate assay uses a single Working Reagent that combines pyruvate oxidase and hydrogen peroxide determination in one step. The color intensity of the reaction product at 570nm or fluorescence intensity at lambda ex/em = 530/585nm is directly proportional to pyruvate concentration in the sample.

-20°C

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